preparation and biodistribution study of 67ga-gallium calcitonin
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abstract
introduction: in order to develop a radiolabeled calcitonin (ct) derivative for receptor imaging studies, ct was successively labeled with 67ga-gallium chloride. methods: the best results of the conjugation were obtained by the addition of 0.5 ml of a ct nasal pharmaceutical solution (1100 iu) to a glass tube pre-coated with dtpa-dianhydride (0.01 mg) at 25°c with continuous mild stirring for 30 min. results: after solid phase purification of the radiolabeled hormone, instant thin layer chromatography (itlc) showed radiochemical purity of higher than 95 % at optimized conditions (specific activity =67-134 kbq/iu, labeling efficiency 70%). 67ga-dtpa-ct mainly accumulates in the liver. conclusion: preliminary in vivo studies (id/g%) in male wild-type rats showed significant liver uptake of the tracer after 24 hours. 67ga-dtpa-ct can be a suitable probe for biodistribution study of ct receptors in various physiological as well as neoplastic lesions with over-expressed calcitonin receptors.
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Journal title:
iranian journal of nuclear medicinePublisher: tehran university of medical sciences
ISSN 1681-2824
volume 19
issue 2 2011
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